鏈接
· MiraCLEAN® 內毒素清除試劑盒
· Exontrap 克隆質粒
· 成像專用培養皿 CG - 顯微鏡配件
· 用于 DNA 純化的 MobiSpin 柱
GeneTrans 基因傳遞試劑
特性
· 多種細胞系中的高轉染效率
· 許多難以轉染的細胞系效果良好
· 性價比高，使用方便
· 配合 Jurkat 細胞時效率最佳
· 寡核苷酸轉染的理想之選
描述
· GeneTrans 轉染試劑已被開發用于多種細胞類型的高效轉染。還可在許多已知的難以轉染的細胞系中實現良好的轉染效率
· GeneTrans 轉染試劑利用由脂質轉染發明人開發的專屬直接親水性綴合 (DHC) 技術。與其他商用的轉染試劑相比，GeneTrans 含有一種理想的調整混合物（其中含有不同修飾的陽離子脂質）。優化脂質/DNA 復合物的組裝，顯著降低細胞毒性。這確保了穩定和瞬時轉染的結果高效且可重復。此外，細胞損傷的顯著減少和培養基變化的消除使 GeneTrans 轉染試劑成為所有基因表達研究的理想試劑，在這些研究中，其中最佳基因傳遞和細胞轉染后狀態都很重要。此外，它們已被證明是寡核苷酸轉染和轉染特殊細胞系（如 Jurkat 細胞）的理想選擇。
GeneTrans-介導的 DNA
進入細胞。
GeneTrans II 轉染試劑
特性
· 優化的轉染效率
· 對于難以轉染的細胞，結果得到大大改善
· 寡核苷酸轉染的理想之選
· 無培養基變化——有無血清均可方便地進行轉染
· 用于蛋白質表達的細胞系具有可靠的可重復結果
· 適用于懸浮細胞和貼壁細胞
· 最小的細胞毒性
· 適用體外和體內轉染
· 具有許多不同細胞系的優異且可重復的性能
描述
· GeneTrans II 轉染試劑是基因傳遞技術的一大進步，基于明顯改進的配方，可進一步增強 DNA 的穩定性，減少在轉染過程中血清成分的干擾。它首先能夠更有效地穩定和保護 DNA，其次，促進其核吸收進入細胞，使 GeneTrans II 成為一款高效的傳遞工具。另外，用 GeneTrans II 配方生產的脂質體與血清組分的相互作用較低，因此特別適用于含血清的培養基。它包括優化的組件和緩沖液，可以通過眾所周知的難以轉染的細胞系（如 HeLa，K-562 和 PC-12）實現卓越的轉染效率。
· GeneTrans II 是高通量篩選和蛋白質表達應用的理想選擇，與其他業界領先的轉染試劑相比具有明顯的優勢。GeneTrans II 提供兩種稀釋劑（DNA 稀釋劑 A 和 DNA 稀釋劑 B），使您可以更靈活地優化所選細胞系的核酸轉染。
· GeneTrans II 配備了優化特定細胞系轉染條件所需的一切，包括方便的 GeneTrans II 干燥脂質膜，水合緩沖液（0.75 ml 或 1.5 ml）和兩種DNA稀釋劑（A 和 B）：4 ml 或 8 ml）。為了方便您的使用，我們提供了一套針對最常用的細胞系測試優化轉染條件的試驗方案。通過遵循本綜合手冊中的簡單步驟，您可以期望最佳的轉染結果。
GeneTrans II 轉染試劑與領先試劑 L2K 的比較。
訂單信息
· 有關運輸和存儲信息，請點擊訂單號。
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訂單號
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描述
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數量
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價格
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數據表
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0201B
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GeneTrans II 轉染試劑
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75 次反應 (0.75ml)
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PDF
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0202B
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GeneTrans II 轉染試劑
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150 次反應 (1.5ml)
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PDF
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0203B
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GeneTrans II 轉染試劑
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750 次反應 (5 x 1.5ml)
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PDF
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0204B
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GeneTrans II QuickEase 試劑盒
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96 個一次性小瓶
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PDF
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參考文獻
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· Kaluza D, Kroll J, Gesierich S, Yao TP, Boon RA, Hergenreider E, Tjwa M, Rössig L, Seto E, Augustin HG, Zeiher AM, Dimmeler S, Urbich C. Class IIb HDAC6 regulates endothelial cell migration and angiogenesis by deacetylation of cortactin EMBO J., Oct 2011; 30:4142 - 4156.
· Carmona G, Göttig S, Orlandi A, Scheele J, Bäuerle T, Jugold M, Kiessling F, Henschler R, Zeiher AM, Dimmeler S, Chavakis E. Role of the small GTPase Rap1 for integrin activity regulation in endothelial cells and angiogenesis Blood, Jan 2009; 113:488 - 497
· Phng LK, Potente M, Leslie JD, Babbage J, Nyqvist D, Lobov I, Ondr JK, Rao S, Lang RA, Thurston G, Gerhardt H. Nrarp Coordinates Endothelial Notch and Wnt Signaling to Control Vessel Density in Angiogenesis Dev.Cell, Vol 16, 1, Jan 2009; 70–82.
· Schröder K, Kohnen A, Aicher A, Liehn EA, Büchse T, Stein S, Weber C, Dimmeler S, Brandes RP.NADPH Oxidase Nox2 Is Required for Hypoxia-Induced Mobilization of Endothelial Progenitor Cells Circ.Res.2009;105;537-544.
· Urbich C, Rössig L, Kaluza D, Potente M, Boeckel JN, Knau A, Diehl F, Geng JG,Hofmann WK, M. Zeiher AM, Dimmeler S. HDAC5 is a repressor of angiogenesis and determines the angiogenic gene expression pattern of endothelial cells Blood, May 2009; 113:5669 - 5679.
· Martin M, Potente M, Janssens V, Vertommen D, Twizere JC, Rider MH, Goris J, Dimmeler S, Kettmann R, Dequiedt F Protein phosphatase 2A controls the activity of histone deacetylase 7 during T cell apoptosis and angiogenesis PNAS, Mar 2008; 105:4727 - 4732.
· Michaelis UR, Xia N, Barbosa-Sicard E, Falck JR, Fleming I Role of Cytochrome P450 2C Epoxygenases in Hypoxia-Induced Cell Migration and Angiogenesis in Retinal Endothelial Cells Invest.Ophthalmol.Vis.Sci., Mar 2008; 49:1242 - 1247.
· Webler AC, Michaelis UR, Popp R, Barbosa-Sicard E, Murugan A, Falck JR, Fisslthaler B, Fleming I. Epoxyeicosatrienoic acids are part of the VEGF-activated signaling cascade leading to angiogenesis.Am J Physiol Cell Physiol.2008 Nov;295(5):C1292-301
· Webler AC, Popp R, Korff T, Michaelis UR, Urbich C, Busse R, and Fleming I. Cytochrome P450 2C9-Induced Angiogenesis Is Dependent on EphB4 Arterioscler Thromb Vasc Biol, Jun 2008; 28:1123 - 1129.
· Diehl F, Rössig L, Zeiher AM, Dimmeler S, Urbich C The histone methyltransferase MLL is an upstream regulator of endothelial-cell sprout formation Blood, Feb 2007; 109:1472 - 1478.
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· Fleming I, Fisslthaler B, Dixit M, Busse R. Role of PECAM-1 in the shear-stress-induced activation of Akt and the endothelial nitric oxide synthase (eNOS) in endothelial cells J. Cell Sci., Sep 2005; 118:4103 - 4111.
· Michaelis UR, Fisslthaler B, Barbosa-Sicard E, Falck JR, Fleming I, Busse R Cytochrome P450 epoxygenases 2C8 and 2C9 are implicated in hypoxia-induced endothelial cell migration and angiogenesis J. Cell Sci., Dec 2005; 118:5489 - 5498.
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